Figure 2.

HMGB1 expression is up-regulated in hepatocytes by hypoxia and in the liver after I/R. (a) Mice underwent 60 min of ischemia and various lengths of reperfusion. Western blot analysis for cellular HMGB1 was performed for hepatic protein lysates of the ischemic lobes at the time points shown, with each lane representing a separate animal. Blot shown is representative of three experiments with similar results. (b) Immunofluorescent stain of HMGB1 from sections of normal liver and (c) liver subjected to 60 min of ischemia and 6 h of reperfusion (original magnification ×400). Images are representative liver sections from six mice per group. Red, HMGB1; blue, nuclei; green, F-actin. (d) Cultured rat hepatocytes were exposed to hypoxia (1% O₂) from 0 to 24 h. Whole cell lystate and media were subjected to Western blot analysis of HMGB1. Blot shown is representative of three experiments with similar results.