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. 2005 Apr 18;201(8):1307–1318. doi: 10.1084/jem.20041385

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Influence of AMD3100, alone and with G-CSF, on circulating HPCs in healthy human volunteers. (A) Influence of multiple injections of AMD3100 alone. Three volunteers were injected i.v. with AMD3100 (80 μg/kg) on the first day (day 1) and again at 24 h. Progenitors/ml blood were assessed preinjection (day 1, 0 h) and at the noted time intervals. Fold changes are calculated based on the following day 1, 0 h progenitors/ml for three donors: CFU-GM (387, 78, 201), BFU-E (712, 78, 217), and CFU-GEMM (77, 29, 93) grown in methylcellulose cultures in the presence of Epo, stem cell factor, and interleukin-3. The fold changes for CFU-GM also include progenitor cells/ml as calculated in agar culture medium with GM-CSF and stem cell factor (control numbers for three donors: 108, 18, 54). *P < 0.001 compared with 0 h counts of that particular day. (B) Influence of AMD3100 on mobilization of circulating HPCs in healthy human volunteers receiving G-CSF or G-CSF plus AMD3100 (160 μg/kg). The results shown are the mean plus range of fold increases of the absolute numbers of CFU-GM and CFU-GEMM per ml of blood for two healthy volunteers each without apheresis. Fold changes are based on the following numbers of G-CSF day 5, 0 h control progenitor cells/ml: group I: G-CSF (CFU-GM: 34403 and 27194; CFU-GEMM: 14363 and 10083); group II: G-CSF (4 d) plus AMD3100 (day 5; CFU-GM: 11968 and 2675; CFU-GEMM: 3366 and 510), and group III: G-CSF (5 d) plus AMD3100 (day 5; CFU-GM: 2698 and 2380; CFU-GEMM: 1028 and 1238). (C) Results of apheresis after mobilization of circulating HPCs. Results are expressed as total progenitors collected (in thousands) for donors receiving G-CSF (n = 3), G-CSF + AMD3100 (160 μg/kg; n = 3), or AMD3100 (240 μg/kg; n = 4). *P < 0.01 for G-CSF– or AMD3100 versus G-CSF + AMD3100-mobilized cells. (D) Results of apheresis shown in C but expressed as progenitors mobilized per kg donor. *P < 0.05 for same comparisons as in C.