Figure 1.

ChA6 mAb inhibits polyclonal proliferation of CD4⁺T cells. (A) CD4⁺ T cells were stimulated with the indicated concentrations of coated anti-CD3 mAb in the presence or absence of chA6 or chimeric isotype control mAb (10 μg/ml) with or without soluble anti-CD28 mAb (1 μg/ml). Results from one representative donor out of four are shown. (B) Dose-dependent inhibition of proliferative polyclonal T cell response by chA6 mAb. CD4⁺ T cells were stimulated with 0.1 and 0.01 μg/ml of coated anti-CD3 mAb in the presence of the indicated concentrations of soluble chA6 mAb. Results from one representative donor out of three (0.1 μg/ml of anti-CD3 mAb) and out of two (0.01 μg/ml of anti-CD3 mAb) are shown. The percentages of inhibition of proliferation in the presence of chA6 mAb relative to control are indicated.