Table II.
Pattern of nucleotide changes in JH4 intronic sequences of normal, polη-, and MSH2-deficient mice
| Within G/C
|
Within A/T
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Trans.
|
Transv.
|
Trans.
|
Transv.
|
|||||
| GC:AT | Transitions: transversions |
G/AC/T | G/TC/A | G/CC/G | A/GT/C | A/TT/A | A/CT/G | |
| Mouse | ||||||||
| Controls 1a | 44.3:55.7 | 53.7:46.3 | 61.4 | 18.5 | 20.1 | 47.6 | 26.2 | 26.2 |
| Controls 1b | 49.9:50.1 | 53.3:46.7 | 54.5 | 16.8 | 28.7 | 52.1 | 25.9 | 22.0 |
| Polη−/− c | 85.5:14.5 | 51.3:48.7 | 57.1 | 12.7 | 30.2 | 17.2 | 26.2 | 56.2 |
| Msh2 −/− d | 87.0:13.0 | 71.2:28.8 | 74.0 | 14.0 | 12.0 | 52.7 | 25.7 | 21.6 |
| Human | ||||||||
| Controls 1e | 46.0:54.0 | 51.2:48.8 | 55.4 | 16.5 | 28.1 | 47.6 | 25.0 | 27.4 |
| XP-V e | 89.3:10.7 | 46.6:53.4 | 51.1 | 17.1 | 31.8 | 9.4 | 36.4 | 54.2 |
Values in bold represent significant differences from controls discussed in the text.
a
This study (122 mutations).
b
From a larger sample (334 mutations; reference 36).
c
This study, data from all deficient mice together (366 mutations).
d
Data from mice deficient in the MSH2-dependent pathway (Msh2 −/−, Exo1 −/−, Msh2 G674A) were pooled (326 mutations obtained by Pfu polymerase; references 8, 11, 12).
e
From ref. 17 (168 mutations).