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. 2005 Oct 3;202(7):975–986. doi: 10.1084/jem.20050421

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — LPA₂ and NHERF2 are expressed in epithelial cells and localized primarily at the apical cell surfaces. (A) LPA₂ expression in crude plasma membranes prepared from HT29-CL19A and Calu-3 cells. (B) Apical localization of LPA₂, CFTR, and ezrin in polarized HT29-CL19A cells by confocal microscopy. AP, apical; BL, basolateral. (C) Expression of LPA₂ and CFTR in apical membranes of HT29-CL19A cells by surface biotinylation method. Smaller amounts of LPA₂ also were observed in the basolateral membrane. (D) NHERF2 expression and apical localization in epithelial cells. The nucleus was stained with propidium iodide solution (bottom).