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. 2005 Oct 3;202(7):975–986. doi: 10.1084/jem.20050421

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — LPA₂ forms a macromolecular complex with CFTR mediated through NHERF2. (A) Pictorial representation of the macromolecular complex assay (see Materials and methods for details). (B) Macromolecular complex of MBP-C–CFTR, GST–NHERF2, and Flag-tagged LPA₂. (C) Macromolecular complex of MBP-C–CFTR, GST–NHERF2, and Flag-tagged LPA₂, LPA₂–ΔSTL, or LPA₂–L351A. (D) BHK or BHK–CFTR cells transiently transfected with Flag-LPA₂ were cross-linked using 1 mM DSP and coimmunoprecipitated using anti-CFTR antibody (NBD-R) and probed for LPA₂. (E) Cell lysates from HT29-CL19A (left) and Calu-3 (right) were coimmunoprecipitated using anti-CFTR antibody (R1104) and probed for NHERF2 and LPA₂. LPA₂ blot was visualized using SuperSignal West Femto Maximum Sensitivity Substrate because of the weak signal (Pierce Chemical Co.).