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. 2005 Oct 3;202(7):975–986. doi: 10.1084/jem.20050421

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — Disruption of the macromolecular complex of LPA₂-NHERF2–CFTR using a LPA₂-specific peptide prevents LPA₂-mediated CFTR inhibition by LPA. (A) I_sc in response to ADO in HT29-CL19A cells with or without LPA₂-specific peptide delivered before I_sc measurement. Cells in both groups were pretreated with LPA 20:4 (25 μM) for 30 min before activating with ADO. (B) Immunofluorescence micrographs of HT29-CL19A cells from (A) showing the LPA₂-specific peptide delivered (top; green). The nucleus was stained with propidium iodide (red). HT29-CL19A cells express endogenous LPA₂ (bottom). (C) NHERF2 binding to LPA₂ in the presence of LPA₂-specific peptide. A curve showing the averaged band density also is presented. *P < 0.05; **P < 0.01 compared with control (n = 3).