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. 2005 Oct 3;202(7):893–900. doi: 10.1084/jem.20051128

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — SLP-76-deficient DP thymocytes cannot transduce TCR-mediated signals. (A). TCR-induced calcium flux was measured in DP-gated thymocytes as described in Materials and methods. (B) Representative FACS of thymocytes from 11-wk-old mice stained with HSA and TCR β. Numbers represent the percentage of cells in the indicated regions. (C) Representative FACS of DP-gated thymocytes from SLP-76^F/FCD4cre and control mice. (D) Thymocytes were left unstimulated or were stimulated with plate-bound anti-CD3, plate-bound CD3 plus CD28, or dexamethasone for 14 h. Acquired cells were gated on DP cells and assessed for exclusion of TOPRO-3. The percentage of TOPRO-3⁻ DP cells relative to total DP cells is shown as percent viable. The reduction in viability of control cells stimulated with CD3 plus CD28 was statistically significant (*P < 0.05). (E) Thymocytes were left unstimulated or were stimulated for 2 h then stained for CD4, CD8, and nur77. Line graphs are gated on DP cells.