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. 2005 Oct 3;202(7):893–900. doi: 10.1084/jem.20051128

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Post DP T cells have an altered CD4/CD8 ratio and cannot flux calcium in response to TCR cross-linking. Representative FACS analysis of (A) HSA^lo-gated thymocytes, (B) live-gated lymph node cells, and (C) CD4-gated splenocytes. Relative percentages of cells within the gated regions are shown. (D) Intracellular staining for SLP-76 and TCR-β in CD4SP thymocytes. Staining controls are mature B220⁺ cells with SLP-76 antiserum and PE-labeled isotype control for TCR-β. (E) TCR-induced calcium flux from SLP-76^F/FCD4cre mice and control animals was performed in CD4SP-gated cells as described in Materials and methods.