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. 2005 May 2;201(9):1421–1433. doi: 10.1084/jem.20042294

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Ly49D-mediated chemokine production requires CD45. (A) IL-15–expanded CD45^−/− and control NK1.1⁺CD49b⁺TCR-β⁻ cells were cultured for 24 h in the presence of plate-bound anti-Ly49D. Culture supernatants were assayed for MIP-1α (*, P = 0.000027) and –1β (**, P = 0.00014) by ELISA. Data from five independent experiments are shown. (B) NK cells were treated with a titration of PP2 and cultured for 24 h in the presence of plate-bound anti-Ly49D. Supernatants were assayed for MIP-1α (*, P = 0.00044) and –1β (**, P= 0.0025) by ELISA. Statistics are for 10 μM PP2 treatment. Data shown are representative of three independent experiments. (C) IL-15–expanded CD45 ⁻ ^/ ⁻, control and PP2-treated NK1.1⁺CD49b⁺TCR-β⁻ cells were cultured for 24 h in the presence of plate-bound anti-Ly49D. Supernatants were assayed for RANTES (*, P = 0.00013; **, P < 0.01) by ELISA. Data are the average of four independent experiments.