Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 May 2;201(9):1367–1373. doi: 10.1084/jem.20050572

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Transcriptional repression of centromeric satellite repeats and features of facultative heterochromatin are maintained in the absence of Dicer. (a) RT-PCR (controlled by GAPDH) did not detect transcripts of major and minor satellite repeat in wild-type (C57BL/10), Dicer ^lox/lox or lckCre Dicer ^Δ/Δ thymocytes, but did in differentiating myoblasts. White lines indicate that intervening lanes have been spliced out. (b) RNA FISH using Xist and Pgk probes of female (XX) control fibroblasts and Dicer ^lox/lox and lckCre Dicer ^Δ/Δ DP thymocytes (see Fig. S2) shows monoallelic, but not biallelic, expression of Pgk. Pgk signals did not overlap with Xist signals. (c) DNA from control XY cells and XX Dicer ^lox/lox or lckCre Dicer ^Δ/Δ thymocytes digested with Xba1/Nru1 (MeCp2) or Xba1/Mlu1 (Mtm1r), and probed for MeCp2 and Mtm1r CpG islands. Upper bands correspond to the methylated (inactive X) allele and lower bands to the unmethylated (active X) allele.