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. 2005 May 2;201(9):1367–1373. doi: 10.1084/jem.20050572

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — CD4/CD8 lineage choice, lineage-appropriate gene expression, and developmentally regulated gene silencing in the absence of Dicer. Similar percentages of Dicer ^lox/lox and lckCre Dicer ^Δ/Δ thymocytes up-regulate CD5 and CD69 at the DP stage (a) and form transitional subsets that are defined by CD4 and CD8 expression (b, mean ± SD, n = 6, see Fig. 2 a), and the expression of CD69 and HSA follows the expected developmental sequence (c). (d) Real-time RT-PCR analysis of CD4 and CD8 lineage-specific transcripts in sorted DP, CD4 SP, and mature (TCR^hi) CD8 SP thymocytes normalized to UBC and YWHAZ control loci (mean ± SD, n = 3). (e) Real-time RT-PCR analysis of Tdt expression ex vivo, 10 h after phorbol ester and ionomycin stimulation (PMA+iono), or 10-h stimulation and 10-h recovery in fresh medium (normalized to UBC and YWHAZ, mean ± SEM, n = 2).

Figure 5. — CD4/CD8 lineage choice, lineage-appropriate gene expression, and developmentally regulated gene silencing in the absence of Dicer. Similar percentages of Dicer ^lox/lox and lckCre Dicer ^Δ/Δ thymocytes up-regulate CD5 and CD69 at the DP stage (a) and form transitional subsets that are defined by CD4 and CD8 expression (b, mean ± SD, n = 6, see Fig. 2 a), and the expression of CD69 and HSA follows the expected developmental sequence (c). (d) Real-time RT-PCR analysis of CD4 and CD8 lineage-specific transcripts in sorted DP, CD4 SP, and mature (TCR^hi) CD8 SP thymocytes normalized to UBC and YWHAZ control loci (mean ± SD, n = 3). (e) Real-time RT-PCR analysis of Tdt expression ex vivo, 10 h after phorbol ester and ionomycin stimulation (PMA+iono), or 10-h stimulation and 10-h recovery in fresh medium (normalized to UBC and YWHAZ, mean ± SEM, n = 2).