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. 2005 Oct 17;202(8):1087–1098. doi: 10.1084/jem.20042530

Figure 5.

Figure 5.

CCR5 deficiency affects immune responses to WNV selectively in the brain. (A) Serum cytokine analysis. The factor analyzed is identified at the bottom of the graph. Data were pooled from two experiments with five to seven mice in each group, and are presented as mean ± SEM pg/ml serum as a function of time after infection. (B and C) Intracellular cytokine staining for WNV-induced IFN-γ. Splenocytes were isolated from mice at the indicated days after WNV infection and assayed without exogenous stimulation (B) or after 4 h of stimulation with ionomycin and PMA (C). Data presented are from one experiment with five mice at each time point. Values at the top of each bar are the percentage of total cells represented by the indicated cell type. (D) Adoptive transfer of CCR5+/+ and CCR5−/− splenocytes from WNV-infected mice into WNV-infected CCR5−/− mice. Infected CCR5−/− recipient mice, designated (WT → KO) and (KO → KO) received splenocytes from infected CCR5+/+ or infected CCR5−/− donor mice, respectively, 4 d after s.c. infection. FACS analysis was performed 4 d after transfer (8 d after infection). Data are presented as the mean ± SEM and are from one experiment with five mice in each group. (B and C) Percents shown are the percentage of total cells. *, P < 0.02 for the indicated comparison (of total cell number) by Student's t test.