Table II.
Effect of CCR5 inactivation on induction of immunoregulatory factors in the brain by WNV
| DAI | Infection | Strain | IL-1β | IL-4 | IL-10 | IFN-α | IFN-γ | TNF-α | ICAM-1 | MMP-9 | TIMP-1 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 | mock | CCR5+/+ | 0.04 ± 0.02 | BLD | BLD | BLD | BLD | BLD | 14.6 ± 0.5 | 4.8 ± 0.6 | 0.4 ± 0.03 |
| 7 | mock | CCR5−/− | 0.2 ± 0.02 | BLD | BLD | BLD | 0.07 ± 0.01 | BLD | 10.6 ± 0.3 | 4.1 ± 0.4 | 0.4 ± 0.04 |
| 7 | WNV | CCR5+/+ | 0.3 ± 0.01 | BLD | BLD | 9.0 ± 2.0 | 0.08 ± 0.05 | 0.7 ± 0.3 | 47.5 ± 7.6 | 53.9 ± 10.1 | 11.8 ± 3.0 |
| 7 | WNV | CCR5−/− | 0.2 ± 0.07 | BLD | BLD | 6.0 ± 2.1 | 0.14 ± 0.08 | 0.3 ± 0.2 | 41.1 ± 7.3 | 28.9 ± 7.6 | 10.9 ± 3.2 |
| 12 | WNV | CCR5+/+ | 0.3 ± 0.08 | BLD | BLD | 0.2 ± 0.1 | 0.09 ± 0.07 | BLD | 41.2 ± 3.7 | 25.1 ± 12.8 | 9.1 ± 1.7 |
| 12 | WNV | CCR5−/− | 0.2 ± 0.07 | BLD | BLD | 0.1 ± 0.07 | 0.06 ± 0.05 | BLD | 29.5 ± 8.5 | 12.5 ± 4.2 | 1.3 ± 0.01a |
Data are pooled from two to three individual experiments with two to three mice in each group and are presented as the mean ± SEM. Units are expressed as nanograms per gram of brain tissue.
a
P < 0.02 when compared to CCR5+/+ mice at the same day after infection.
BLD; below the level of detection; DAI, days after infection.