Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Nov 7;202(9):1213–1223. doi: 10.1084/jem.20051135

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — TSLP-DCs express OX40L. (A) Each column represents data from human blood CD11c⁺ immature mDCs either resting or activated by poly I:C or TSLP. The selected 2,166 genes were grouped, based on similarity in expression patterns, by hierarchical clustering as described in Materials and methods. Each row represents relative hybridization intensities of a particular gene across different samples. Colors reflect the magnitude of relative expression of a particular gene across samples. Cluster I, II, and III include genes highly expressed in poly I:C–activated DCs, resting DCs, and TSLP-activated DCs, respectively. Cluster IV includes genes highly expressed in both poly I:C–activated and TSLP-activated DCs. Under culture with different stimuli, expression profiles of the indicated genes (B) and OX40L mRNA expression (C) in DCs were analyzed at the 24-h time point by microarray and RT-PCR, respectively, and surface expression of OX40L on DCs were analyzed at the 48-h time point by flow cytometry (D). OX40L expression on TSLP-DCs were monitored at different time points (E). The staining profile of anti-OX40L mAb and isotype-matched control are shown by the shaded and open areas, respectively. The results of the gene expression profiles (B) are shown as the relative hybridization intensity level by microarray analysis. Accession number of each microarray dataset are available at http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene.