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. 2005 Nov 7;202(9):1213–1223. doi: 10.1084/jem.20051135

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — TSLP-DC-mediated inflammatory Th2 cell response requires OX40L as a positive Th2 cell–polarizing signal and default of a lack of IL-12. CD4⁺ naive T cells were cocultured with med-DCs (A), poly I:C-DCs (B), or TSLP-DCs (C and D) for 7 d in the presence of the indicated neutralizing Abs or recombinant IL-12. Cytokine production by T cells was analyzed intracellularly by flow cytometry (A–C) and measured in supernatants after restimulation with anti-CD3 and anti-CD28 mAbs for 24 h by ELISA (D). Percentages of the respective cytokine-producing T cells are indicated in each dot blot profile in A–C. Error bars in D represent standard deviations of triplicate cultures. Data are from one of four independent experiments.