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. 2005 Nov 7;202(9):1213–1223. doi: 10.1084/jem.20051135

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — OX40L-transfected L cells enhance TNF-α production and inhibit IL-10 production in T cells. CD4⁺ naive T cells were cultured with anti-CD3 and anti-CD28 mAbs on OX40L-transfected L cells and/or the indicated recombinant cytokines for 7 d. Cytokine production by CD4⁺ T cells was analyzed intracellularly by flow cytometry (A) and measured in supernatants after restimulation with anti-CD3 and anti-CD28 mAbs for 24 h by ELISA (B). Percentages of the respective cytokine-producing T cells are indicated in each dot blot profile in A. Error bars in B represent standard deviations of triplicate cultures. Data are from one of four independent experiments.