Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jun 6;201(11):1805–1814. doi: 10.1084/jem.20050011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Motility of auto and control antigen-specific T cells in EAE lesions. (A, B) Two-photon live microscopy of T_MBP-GFP cells in acute spinal cord slices. Two types of T cell movements are observed within CNS lesions: “motile” (A) and “stationary” (B) T_MBP-GFP cells. Numbers indicate time after start of the analysis (minutes). Dotted lines indicate the trajectories and the cell shape of the preceding pictures. Arrows point to the tracked cells. Representative cells of 10 videos from four independent experiments. Bars, 10 μm. (C) Locomotion of T_OVA-GFP cells in living spinal cord slices analyzed by two-photon analysis (4 d after cotransfer of T_MBP cells and T_OVA-GFP cells). A representative cell of 6 videos from three independent experiments is shown. Bar, 10 μm. (D) Proportion of motile versus stationary MBP- (T_MBP cells) and OVA- (T_OVA cells) specific T cells in living CNS slices (video microscopy). Motile T cells were defined as cells which migrated >10 μm in 10 min. Cells were grouped as stationary if they moved <10 μm in 10 min. T_MBP cells: means ± SD from 6 independent experiments including >700 cells from 11 videos. T_OVA cells: means ± SD of 187 cells from 4 videos and three independent experiments. Student's t test, *P < 0.001.

Figure 2. — Motility of auto and control antigen-specific T cells in EAE lesions. (A, B) Two-photon live microscopy of T_MBP-GFP cells in acute spinal cord slices. Two types of T cell movements are observed within CNS lesions: “motile” (A) and “stationary” (B) T_MBP-GFP cells. Numbers indicate time after start of the analysis (minutes). Dotted lines indicate the trajectories and the cell shape of the preceding pictures. Arrows point to the tracked cells. Representative cells of 10 videos from four independent experiments. Bars, 10 μm. (C) Locomotion of T_OVA-GFP cells in living spinal cord slices analyzed by two-photon analysis (4 d after cotransfer of T_MBP cells and T_OVA-GFP cells). A representative cell of 6 videos from three independent experiments is shown. Bar, 10 μm. (D) Proportion of motile versus stationary MBP- (T_MBP cells) and OVA- (T_OVA cells) specific T cells in living CNS slices (video microscopy). Motile T cells were defined as cells which migrated >10 μm in 10 min. Cells were grouped as stationary if they moved <10 μm in 10 min. T_MBP cells: means ± SD from 6 independent experiments including >700 cells from 11 videos. T_OVA cells: means ± SD of 187 cells from 4 videos and three independent experiments. Student's t test, *P < 0.001.