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. 2005 Jun 6;201(11):1805–1814. doi: 10.1084/jem.20050011

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — Immune synapses-like membrane structures in the CNS. (A) Polarization of TCR/LFA-1 in T_MBP-GFP cells 4 d after transfer in spinal cord slices. Confocal imaging, immunostaining with TCR/LFA-1. GFP (green), LFA-1 (red), TCR (blue). T_MBP-GFP cells with polarized (closed arrow) and evenly distributed (open arrow) TCR/LFA-1 pattern. (B) Three-dimensional reconstruction of the synapse-like–forming T_MBP-GFP cell (indicated cell from Fig. 6 A). (C) Contact plane of a T_MBP-GFP cell with synapse-like TCR/LFA-1 polarization (arrow). (D) T_MBP-GFP cell (green) contacting a MHC class II⁺ cell (red). Note the polarization of TCR (blue) at the contact point of the cells. (E) T_MBP-GFP cells that form synapse-like TCR/LFA-1 polarizations are “stationary.” Fluorescence video microscopy of living spinal cord tissue 4 d after transfer stained with anti-TCR/LFA-1 antibodies. Numbers indicate the time points of image acquisition. Overlay of GFP (green), TCR (red), and LFA1 (blue) is shown. Bars, 10 μm.