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. 2005 Jun 6;201(11):1853–1863. doi: 10.1084/jem.20041392

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — SURFIN accumulates within the parasitophorous vacuole in late schizonts and is associated with released merozoites. (A) Alexa 488–labeled SURFIN_4.2 (green) detected with anti-S1.3 antibodies in parasites developing from late trophozoite stage to schizonts. PI staining (red) was used to visualize nuclei. (B–D) SURFIN_4.2 was found localized in MAM and the PM of late and bursting schizonts (>48 h), and associated with the released merozoite surface. Dashed circles indicate the area of the IE. (B) IFA with anti-S1.3 + PI on bursting schizonts. IFA (anti-rSURFIN_4.2) and immunoelectron microscopy (anti-S1.4 Ig) on late schizonts (C) and released merozoites (D). SURFIN associated with PM or MAM is indicated by arrows. (E) SURFIN_4.2 did not colocalize with the microneme AMA-1. A detailed analysis of SURFIN_4.2 (anti-S1.3 antibodies) and AMA-1 in relation to the nuclei and to each other in merozoites is shown. (B and E) A schematic representation of the antigen localization is shown on the right. Bars: IFA, 2 μm; EM, 0.5.