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. 2004 Mar 1;199(5):687–695. doi: 10.1084/jem.20031636

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — CHIPS binding to neutrophils of different species. (A) Leukocytes of different species were incubated with 1 μg/ml CHIPS-FITC and analyzed by flow cytometry based on scatter parameters to identify neutrophils. Data shown are from one representative experiment. (B) The effect of different concentrations CHIPS on the C5a-induced calcium mobilization in human (♦) and mouse (•) neutrophils. Data are expressed as percentage inhibition of the response of control cells to 10⁻¹⁰ M C5a (n = 2). The response was defined by the difference in mean fluorescence (MFL) of basal level and maximal level after challenge (ΔMFL = 84.3 for human and 22.9 for mouse).