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. 2004 Jul 5;200(1):13–24. doi: 10.1084/jem.20031680

Figure 6.

Figure 6.

Expression of the HSV TAP inhibitor ICP47 differentially impairs cell surface expression of HLA-B*4402 versus B*4405. (A) Cell surface expression HLA-B*4402 and B*4405 by C1R transfectants in the presence and absence of ICP47. Cells were stained by indirect immunofluorescence for the shared Bw4 determinant after culture at 37°C (bold dotted lines) or 26°C (solid lines). Flow cytometric histograms include staining of untransfected cells (thin dotted lines) and conjugate alone (filled histograms). (B) Steady-state total HLA–class I heavy chain expression in C1R transfectants ± ICP47. Immunoblots using mAb HC-10 were performed on graded cell numbers of transfectants. (C) Lack of detectable B*4405 within the TAP-associated PLC of C1R transfectants expressing ICP47. TAP complexes were immunoprecipitated from C1R transfectants expressing either B*4402 or B*4405 ± ICP47 using mAb 148.3. Immunoprecipitates were subsequently immunoblotted for the presence of class I hc using biotinylated HC-10 and developed as described in Fig. 2.