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. 2004 Jul 5;200(1):99–106. doi: 10.1084/jem.20032100

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Effect of inhibition of apoptotic pathways on PtdCho synthesis inhibition by S. pneumoniae. Microglia were treated with the indicated inhibitor and then exposed to bacteria for 4 h. [methyl-³H]choline incorporation was quantified by scintillation counting and apoptosis was determined by annexin V labeling. Values were compared with control, untreated cells (29,221 dpm/mg protein = 100%). ZVAD, Z-Val_Ala_Asp(Ome)-Fmk; ALLN, N-acetyl-Leu-Leu-NLE-CHO; BAPTA, Ca₂- chelator. *, P < 0.05 comparing inhibition of labeling in infected cells plus inhibitor to control cells plus inhibitor. There is no significant difference between values shown and infected cells without inhibitor.