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. 2005 Dec 5;202(11):1599–1611. doi: 10.1084/jem.20050967

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Cell cycle status in the pool of multipotent bone marrow cells. (A) FACS sorting conditions used to purify LT-HSC, ST-HSC^F, and MPP^F. (B) Cell cycle analyses of purified populations by H/PY (top, contour plot) and PI (bottom, histogram) staining. The percentages of cells in G₀ (H^2N/PY⁻), G₁ (H^2N/PY⁺), or S-G₂/M (H^>2N-4N/PY⁺) and in sub2N (<2N), G₀/G₁ (=2N), S (>2N and <4N), and/or G₂/M (=4N) are indicated. (C) Scheme of the BrdU incorporation experiment. 1 mg BrdU (n = 20 mice/time point) was injected intraperitoneally at the start of the kinetic (t = 0) and every 6 h thereafter, and BM cells were harvested after 1 h (t = 1 h; one injection of BrdU), 12 h (t = 12 h; two injections of BrdU), and 24 h (t = 24 h; four injections of BrdU). (D) Short-term kinetics of BrdU incorporation. At the indicated time after BrdU injection, each population was purified by double FACS sorting, and analyzed by flow cytometry for BrdU/PI staining (dot plots). The percentage of BrdU⁺ cells is indicated (*, potential background). Histograms (right) indicate the changes over time in BrdU fluorescence intensity.