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. 2005 Dec 5;202(11):1599–1611. doi: 10.1084/jem.20050967

Table II.

Engraftment capacity of mobilized BM LT-HSC

4 wk
8 wk
12–18 wk
Cell # Frequencyreconstitution(#/total) Average chimerism(range)engraftment type(#/reconstituted) Frequencyreconstitution(#/total) Average chimerism(range)engraftment type(#/reconstituted) Frequencyreconstitution(#/total) Average chimerism(range)engraftment type(#/reconstituted)
Untreated
BM LT-HSC
50 100% (8/8) 11.07% (2.3–22.7%)
M + L (8/8)
100% (7/7) 29.2% (6.7–56.9%)
M + L (7/7)
100% (7/7) 30.2% (6.2–56.2%)
M + L (7/7)
D2 Cy/G mobilized
BM LT-HSC
50 46% (7/15) 2.9% (0.2–12.5%)
M + L (7/7)
40% (6/15) 6.1% (0.4–26.8%)
M + L (4/6), L only (2/6)
40% (6/15) 4.6% (0.1–18.1%)
M + L (4/6), L only (2/6)

LT-HSC (Lin/c-Kit+/Sca-1+/Thy1.1int/Flk-2) were double FACS–sorted from BM of untreated C57BL/6-Ly5.1 donor mice or from mice subjected to the D2 Cy/G mobilization treatment and tested for their engraftment capacities by limited dilution competitive reconstitution assays. 50 cells were transplanted into lethally irradiated congenic C57BL/6-Ly5.2 recipient mice together with 3 × 105 Ly5.2+ helper BM cells. Recipient mice were considered to be reconstituted by Ly5.1+ donor cells if the frequencies of Ly5.1+ donor-derived myeloid (M, Gr-1+, or Mac-1+) or lymphoid (L, B220+, or CD3+) cells detected in the peripheral blood at the indicated times after transplantation were greater than background levels (≥0.1% as determined by parallel analysis of untransplanted control C57BL/6-Ly5.2 mice). M + L indicates multilineage reconstitution.