Figure 9.

Influence of S-(4-Nitrobenzyl)-6-thioinosine (NBTI) on pulmonary edema, vascular permeability, and PMN accumulation in vivo. BL/6/129 mice were administered NBTI (1 mg/kg i.p. in DMSO) or vehicle alone, and exposed to normoxia or hypoxia for 4 h. (a) Assessment of lung water content in normoxia (black bars) and hypoxia (gray bars) after dipyridamole or vehicle treatment. Data are expressed as mean ± SD mg H₂O/mg dry tissue, and are pooled from four animals per condition. *, difference between hypoxia and normoxia (P < 0.025); ^#, difference between dipyridamole treatment and vehicle control (P < 0.025). (b) To assess vascular barrier function, animals were administered intravenous Evan's blue solution (0.2 ml of 0.5% in PBS) before normoxia/hypoxia exposure. Animals were killed and indicated organs were harvested. Evan's blue concentrations were quantified as described in Materials and methods. Data are expressed as mean ± SD Evan's blue OD/50 mg wet tissue and are pooled from four to six animals per condition. *, differences between NBTI/vehicle treatment groups (P < 0.025); ^#, differences between normoxia/hypoxia exposure (P < 0.05). (c) Organ assessment of PMN accumulation by MPO measurements in the indicated organs after 4 h of normoxia/hypoxia exposure (*P < 0.025 compared with vehicle; ^#P < 0.025 compared with normoxia).