Figure 7.

1018 ISS prevents allergen-induced Th2 and Th2-dependent gene induction at an early time point after challenge. mRNA expression measured in whole lung is shown. Mice were sensitized with RW and alum on days 0 and 3. Lungs were harvested 6 h after intratracheal challenge (day 10) with 200 μg RW in sensitized mice. 1 d before the RW challenge, mice were given PBS (black bars), 20 μg 1018 ISS (gray bars), or control ODN (not depicted) intratracheally. Mice challenged with PBS only (and not with RW) are represented by white bars. All data are expressed as gene/ubiquitin ratio (mean ± SEM; five mice per group), and * indicates a p-value of <0.05 when compared with the PBS-only challenged group. RW challenge in sensitized mice induces significant up-regulation of Th2 and Th2-dependent genes 6 h after challenge. Pretreatment with 1018 ISS completely inhibits the induction of these genes, with the exception of IL-4.