Table II.
Autoantigen-precipitating activity of SLE patient sera
Patient | RNP | Sm | Ro60 | La | Core histones | RNAPII | Su | DNA-PK | OJ |
---|---|---|---|---|---|---|---|---|---|
6564 | X | ||||||||
6567 | |||||||||
6569 | |||||||||
6571 | X | X | |||||||
6574 | X | X | X | X | |||||
6575 | X | ||||||||
6576 | X | ||||||||
6579 | X | X | X | ||||||
6622 | X | X | X | X | |||||
6627 | X | ||||||||
6628 | X | X | |||||||
6630 | X | X | |||||||
6633 | X | ||||||||
6642 | X | X |
Immunoprecipitations were performed as described in Materials and methods, and as shown in Fig. 6 D. X indicates a positive result as defined for the following specificities: RNP, immunoprecipitation of A, B′/B, C, D, E, F, and G, but not U5 200K protein; Sm, immunoprecipitation of A, B′/B, C, D, E, F, and G plus U5 200K protein; Ro60, immunoprecipitation of 60 kD protein; La, immunoprecipitation of 45 kD protein; Core histones, immunoprecipitation of H2A/H2B plus unidentified band; RNAPII, immunoprecipitation of RNA polymerase II, two largest subunits; DNA-PK, immunoprecipitation of DNA-PKcs, Ku70, and Ku80 proteins; OJ, immunoprecipitation of multiprotein complex.