Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Dec 5;202(11):1549–1561. doi: 10.1084/jem.20051506

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Natural CD4⁺CD25⁺ T reg cells are equally functional in A/J and C3H animals. CD4⁺CD25⁻ and CD4⁺CD25^bright cells were purified from the lungs of HDM-sensitized and -challenged A/J or C3H mice and stained with CFSE. 50,000 CFSE-labeled CD4⁺CD25⁻ T cells were co-cultured with the indicated numbers of CD4⁺CD25^bright and stimulated with anti-CD3/-CD28–coated latex beads as described in Materials and methods. Co-cultures consisted of CD4⁺CD25⁻/CD4⁺CD25^bright population from A/J/A/J, A/J/C3H, C3H/A/J, or C3H/C3H mice as indicated. TCR-β⁺ T cells were detected via flow cytometry, and intensity of CFSE staining was used to identify T cells that remained undivided after 5 d of culture (A). Levels of IL-2 (B), IL-10 (C), or IFN-γ (D) were assessed in tissue culture supernatant by ELISA. Data represent means ± SEM. *, P < 0.05 between cultures stimulated in the presence and absence of CD4⁺CD25^bright.