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. 2005 Dec 5;202(11):1471–1476. doi: 10.1084/jem.20051611

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Loss of Blimp-1 in vitro. Splenocytes were treated with LPS for 3 d in vitro followed by 4 d of 4-hydroxytamoxifen (4OHT) or sham treatment. (A) Western blot for Blimp-1 and actin protein in ERCre ⁺ prdm1 ^F/F and control cultures treated with 4OHT and sham. (B) CD138 vs. B220 staining of splenocyte cultures. (left) Control; (right) ERCre ⁺ prdm1 ^F/F cultures; (top) sham; (bottom) 4OHT treated. The frequency of gated CD138^HI plasma cells is indicated. Depicted results are representative of five experiments. (C) Kinetics of plasma cell disappearance after 4OHT treatment. (left) The percent of total B220^LOCD138^HI plasma cells in control (solid square) and ERCre ⁺ prdm1 ^F/F (open square) cultures for 4 d after 4OHT treatment. (right) The percent of CFSE^HI plasma cells in these cultures. (D) IgM-secreting cells as assessed by ELISPOT analysis for ERCre ⁺ prdm1 ^F/F and control cultures treated with 4OHT and sham. The y axis represents number of IgM-secreting cells per 2,000 cells. C and D are representative of two experiments.