Table III.
Cytokine production by Meg2+/+ and Meg2−/− peripheral lymphocytes
| Stimulus | Control | Anti-CD3 | Anti-CD3 + CD28 | Con A | PMA + Ionomycin | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Meg2 Genotype | +/+ | −/− | +/+ | −/− | +/+ | −/− | +/+ | −/− | +/+ | −/− |
| GM-CSF | 43 | 35 | 151 | 82 | 194 | 92 | 84 | 71 | 191 | 90 |
| IFN-γ | nd | nd | 356 | 87 | 451 | 121 | nd | 62 | 141 | 124 |
| TNF-α | nd | nd | 56 | 35 | 62 | 31 | 4 | 26 | 47 | 32 |
| IL-1β | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| IL-4 | nd | nd | 100 | 43 | 129 | 49 | nd | 19 | 31 | 37 |
| IL-5 | nd | nd | 89 | 41 | 117 | 30 | 10 | nd | 18 | 64 |
| IL-6 | 9 | 9 | 761 | 306 | 1,042 | 360 | 51 | 231 | 401 | 376 |
| IL-10 | 9 | 9 | 74 | 37 | 71 | 44 | 13 | 34 | 23 | 34 |
| IL-12 (p70) | 9 | 8 | 13 | 11 | 13 | 10 | 9 | 10 | 12 | 11 |
Data represent extracellular cytokine levels in pg/ml as assessed by a bead binding array. n.d., none detected (below limits of detection). These results are representative of two separate experiments performed in duplicate.