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. 1998 Aug 17;188(4):791–795. doi: 10.1084/jem.188.4.791

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The SLP-65 gene encodes a protein with several regions of similarity to SLP-76 and is transcribed in lymphoid tissues. (A) Deduced amino acid sequence of murine SLP-65. Dots, Potential tyrosine-phosphorylation sites are marked. Box, The COOH-terminal SH2 domain. Underline, Peptide sequences obtained from affinity-purified SLP-65. (B) SLP-65 transcripts of ∼2 kb were detected by Northern blot analysis of RNA from the indicated tissues and cell lines. The SLP-65 probe used was derived from the ORF, and a reference signal for ribosomal 28S RNA was used as a loading control. (C) Anti–SLP-65 precipitates were prepared from postnuclear supernatants of pervanadate/H₂O₂-stimulated J558Lμm3 cells, transiently transfected either with the parental vector (lane 1) or an expression vector encoding SLP-65 with an NH₂-terminal HA tag (lane 2). Precipitated proteins were analyzed by antiphosphotyrosine (anti-pY) immunoblotting.