Figure 2.

(a and b) CD8⁺ lymph node T cells were purified from female Egr/H-Y mice (Egr) on H-2^b/b, H-2^d/d, and β2m⁰ backgrounds and from female NL/H-Y mice (NL) on H-2^b/b by depleting CD4⁺ cells and class II MHC–positive cells. Cells were stimulated by culture with (a) immobilized anti-CD3 mAb or (b) Con A. In both experiments, proliferation was assessed in duplicate samples by [³H]thymidine incorporation. Data are representative of four independent experiments. (c) Purified CD8⁺ T cells including 5 × 10⁶ H-Y CD8⁺ cells in PBS were injected intravenously into male or female Cα^−/− recipient mice. 4 d after injection, the numbers of H-Y CD8⁺ cells in recipient spleens were evaluated and are represented by bars ± SD. Three sets of independent experiments were performed. Numbers of H-Y CD8⁺ cells in female recipients were identical or less than those of mice with no cells injected [donor (−)], which is indicative of background in the fluorocytometric analysis. (d) Purified CD8⁺ T cells including the indicated numbers of H-Y CD8⁺ cells were stimulated by 3,000-rad irradiated spleen cells from male or female B6 mice. Proliferation was assessed by duplicate [³H]thymidine incorporation. Results are representative of three sets of independent experiments; all experiments showed similar results.