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. 1998 Aug 17;188(4):619–626. doi: 10.1084/jem.188.4.619

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Retroviral gene transfer of 3G6-CD28, 3G6-CD28TR, and NTP in human primary lymphocytes. PBLs were stained 72 h after retroviral infection with antibodies to CD8 (x-axis) and either A1G4 mAb to stain 3G6-CD28 and 3G6-CD28TR or 20.4 mAb to stain NTP (y-axis). Staining with secondary antibody alone (GAR-PE; A) indicates the background staining. Staining with A1G4 (antiidiotype; B and C) and with 20.4 (anti– NTP; D), followed by the corresponding PE conjugates.