Figure 5. Bone and cartilaginous skull tissue anatomy of wild-type (+/+) and Eya4^–/– (–/–) newborn mice.

Top (A) and lateral (B) skull views demonstrated no craniofacial abnormalities in mutant or wild-type mice (n = 3 for each genotype), but ventral view (C) showed abnormal fusion of palatal bones (dashed box) in Eya4^–/– mice. (D) Higher magnification of palatal bones (C, dashed box) and schematic detailing morphologic abnormalities. Note wide separation between flanking palatal bones (incomplete fusion) in Eya4^–/– skulls. Pt, pterygoid bone; m, maxillary shelves; P, palate bone. Scale bar: 1 mm.