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. 1998 Sep 7;188(5):931–939. doi: 10.1084/jem.188.5.931

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Inactivation of Rho function inhibits p56lck-initiated pre-T cell proliferation. (A) Inactivation of Rho function during early thymopoiesis prevents hyperproliferative responses in pre-T cells from pLGF transgenic mice. Flow cytometric analysis of cellular DNA content of CD44^neg TN thymocytes from pLGF transgenic, and pLGF/C3 double transgenic mice as described above. (B) Loss of Rho function prevents the accumulation of CD25⁻CD44⁻ pre-T cells in thymocytes expressing active p56lck. Total thymocytes from normal, pLGF transgenic and pLGF/C3 double transgenic mice were prepared and stained with mAb against a panel of mature lineage markers including CD4, CD8, CD3, B220, NK, Gr-1, Mac-1 (all biotinylated), CD25 (FITC), and CD44 (PE). Thymocytes negative for all lineage markers were gated and examined for their CD25/CD44 profiles. Absolute cell numbers in individual CD25/CD44 thymocyte subsets were calculated on the basis of average percentages obtained in these analyses and average numbers of TN thymocytes in pLGF and pLGF/C3 mice. Columns represent means of six mice analyzed, bars indicate standard deviations.

Inactivation of Rho function inhibits p56lck-initiated pre-T cell proliferation. (A) Inactivation of Rho function during early thymopoiesis prevents hyperproliferative responses in pre-T cells from pLGF transgenic mice. Flow cytometric analysis of cellular DNA content of CD44^neg TN thymocytes from pLGF transgenic, and pLGF/C3 double transgenic mice as described above. (B) Loss of Rho function prevents the accumulation of CD25⁻CD44⁻ pre-T cells in thymocytes expressing active p56lck. Total thymocytes from normal, pLGF transgenic and pLGF/C3 double transgenic mice were prepared and stained with mAb against a panel of mature lineage markers including CD4, CD8, CD3, B220, NK, Gr-1, Mac-1 (all biotinylated), CD25 (FITC), and CD44 (PE). Thymocytes negative for all lineage markers were gated and examined for their CD25/CD44 profiles. Absolute cell numbers in individual CD25/CD44 thymocyte subsets were calculated on the basis of average percentages obtained in these analyses and average numbers of TN thymocytes in pLGF and pLGF/C3 mice. Columns represent means of six mice analyzed, bars indicate standard deviations.