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. 1998 Oct 19;188(8):1503–1510. doi: 10.1084/jem.188.8.1503

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BLR1 expression is maintained in the absence of TNF and LT. Spleen cells from WT C57BL/6, TNF^−/−, and TNF/LTα^−/− mice were dual labeled for B cells (PE anti-B220 mAb) and with either rabbit anti-BLR1 or as a control, rabbit IgG (10 μg/ml) followed by FITC anti– rabbit IgG. Histograms, BLR1 expression of B220⁺ cells. Dotted line, Rabbit IgG background staining of WT B220⁺ cells. Background staining of B cells from TNF^−/− and TNF/LTα^−/− mice was comparable to that of WT mice. Spleen cells derived from groups of three mice were examined individually, and similar results were obtained for each.