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. 1998 Oct 19;188(8):1503–1510. doi: 10.1084/jem.188.8.1503

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Activated B cells migrate normally to the outer PALS in TNF^−/− mice. Splenic HEL-Ig Tg B cells were isolated and stimulated in vitro for 60 min with 100 ng/ml HEL. Cells were washed and transferred intravenously into WT, TNF^−/−, and TNF/LTα^−/− recipients. Spleen sections were analyzed 20 h later by staining for B cells (red) and activated HEL-specific Ig Tg B cells (green/yellow). Bar = 200 μm.