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. 1998 Oct 19;188(8):1521–1528. doi: 10.1084/jem.188.8.1521

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NK T cells from human PBMCs expand in response to α-GalCer. Crude PBMCs were cultivated in the presence of 50 U/ml of IL-2 and 100 ng/ml of α-GalCer. At day 10 harvested cells from XC cell line (top) or PB cell line (bottom) were cultured for 5 d in the presence of IL-2 only. Proliferating T cells were then cultured for another 5 d with α-GalCer pulsed C1R-hCD1d transfectants in the presence of 50 U of IL-2. Expansion of the Vα24⁺ cells was determined upon staining with anti-CD4 and anti-Vα24 mAbs at days 0, 9, and 21.