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L. major amastigotes induce upregulation of FSDDC MHC class I, class II, and costimulatory molecule expression. L. major amastigotes, metacyclic promastigotes, or LPS (100 ng/ml) and IFN-γ (1,000 U/ml) were added to FSDDC, and effects on surface Ag expression were assessed using flow cytometry 18 h later. Residual aggregates were dissociated with EDTA before analysis. Shaded areas, mAbs of interest; solid lines, isotype controls (n = 4).
