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L. major does not activate inflammatory Mφ. Polyacrylamide bead–elicited tissue macrophages were infected with L. major amastigotes or metacyclic promastigotes, or stimulated with LPS (100 ng/ml) and IFN-γ (1,000 U/ml) for 18 h. MHC class II, CD40, CD54, and CD86 levels were analyzed after 18 h using flow cytometry. Shaded areas, mAbs of interest; solid lines, isotype control (n = 4).
