Table 1.
Additions |
Cytokines analyzed | |||||||
---|---|---|---|---|---|---|---|---|
IL-12 (p40) | IL-12 (p70) | TNF-α | IL-6 | |||||
FSDDC | ||||||||
None | 34 ± 13 | <5 | <35 | 123 ± 67 | ||||
Metacyclic promastigotes | 28 ± 17 | <5 | <35 | 163 ± 119 | ||||
Amastigotes | 346 ± 134‡ | <5 | 112 ± 43‡ | 178 ± 107 | ||||
LPS + IFN-γ | 1,226 ± 507‡ | <5 | 342 ± 130‡ | 1,599 ± 737‡ | ||||
Amastigotes + LPS + IFN-γ≳ | 2,323 ± 1,584‡ | <5 | 503 ± 56‡ | 969 ± 258‡ | ||||
Mφ | ||||||||
None | 43 ± 11 | <5 | <35 | 1,045 ± 453 | ||||
Metacyclic promastigotes | 27 ± 13 | <5 | <35 | 868 ± 868 | ||||
Amastigotes | 44 ± 29 | <5 | 251 ± 251 | 701 ± 481 | ||||
LPS + IFN-γ | 3,610 ± 1,648 | 686 ± 450 | 5,447 ± 425 | >20,000 | ||||
Amastigotes + LPS + IFN-γ | 1,028 ± 595 | 45 ± 45 | 2,949 ± 1,561 | 11,585 ± 4,902 |
FSDDC aggregates (n = 9) or Mφ (n = 4) were incubated with L. major promastigotes or amastigotes (two to three organisms/cell) or with LPS (100 ng/ml) plus IFN-γ (1,000 U/ml) as indicated. Cytokine levels in 18-h supernatants were determined by ELISA. Values represent pg/ml of cytokine produced by 2 × 105 cells/ml in 18 h (mean ± SEM).
LPS and IFN-γ were added 3 h after amastigotes.
P < 0.05 compared with untreated control.