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. 1998 Oct 19;188(8):1529–1534. doi: 10.1084/jem.188.8.1529

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Requirement for antigen uptake and endosomal delivery of CD1d in recognition of synthetic glycolipids by human NK T cells. (a) Proliferative responses of clone DN2.C7 to ECDI-fixed APCs. CD1d⁺ HeLa cells either were pulsed for 12 h with αGalAPS (100 ng/ml) and subsequently fixed by ECDI treatment, or were fixed first followed by αGalAPS pulsing. White bars represent APCs pulsed with vehicle (DMSO) alone, and black bars represent APCs pulsed with αGalAPS. (b) Proliferation of clone DN2.C7 to αGalAPS presented by HeLa cell transfectants expressing wild-type CD1d (circles) versus HeLa transfectants expressing the CD1d/a chimeric protein that lacks an endosomal targeting signal (squares). Both transfectants expressed comparable levels of immunoreactive CD1d on the cell surface. Proliferation in the absence of APCs was <200 cpm in this experiment. Note that DN2.C7 showed a weak but significant response to the CD1d/a transfectant in the absence of added antigen, but no augmentation of this response at any concentration of αGalAPS tested.