Table 2.
γδ T Cell Hybridomas with Two Complete VDJδ Rearrangements
| Hybridoma | Rearrangement | In-frame | Rearrangement | In-frame | ||||
|---|---|---|---|---|---|---|---|---|
| F1D.9 | DV107S7-Jδ1 | Y | ADV7S2-Jδ1 | N | ||||
| F1D.17 | ADV7S1-Jδ1 | Y | DV105S1-Jδ1 | N | ||||
| F1D.23 | ADV7S1-Jδ1 | Y | DV105S1-Jδ1 | N | ||||
| F1D.29 | ADV7S1-Jδ1 | Y | DV104S1-Jδ1 | N | ||||
| F1D.32 | DV7S-Jδ1 | Y | DV105S1-Jδ1 | Y | ||||
| F1D.33 | ADV7S1-Jδ1 | Y | ADV17S2-Jδ1 | N | ||||
| F1D.36 | DV104S1-Jδ1 | Y | ND | |||||
| F1D.42 | DV10S7-Jδ1 | Y | ADV17S2-Jδ1 | N | ||||
| F1D.49 | DV7S5-Jδ1 | Y | DV104S1-Jδ1 | Y | ||||
| F1D.57 | DV104S1-Jδ1 | Y | DV7S4-Jδ1 | N | ||||
| F1D.60 | ADV7S2-Jδ1 | Y | DV104S1-Jδ1 | Y | ||||
| F1D.61 | DV105S1-Jδ1 | Y | DV104S1-Jδ1 | Y | ||||
| F1D.64 | ND | Y | DV7S6-Jδ1 | N | ||||
| F1D.67 | DV104S1-Jδ1 | Y | DV7S5-Jδ1 | N | ||||
| F1D.73 | DV104S1-Jδ1 | Y | DV104S1-Jδ1 | Y | ||||
| F1D.75 | ADV7S1-Jδ1 | Y | DV104S1-Jδ1 | Y | ||||
| F1D.83 | DV102S1-Jδ1 | Y | DV7S5-Jδ1 | N | ||||
| F1D.89 | DV104S1-Jδ1 | Y | DV7S5-Jδ1 | N | ||||
| F1D.91 | DV7S6-Jδ1 | Y | ND |
F1D.32 has a rearrangement utilizing a DV7S Vδ gene segment that differs from other known family members by at least five nucleotides (data not shown). This Vδ gene segment may represent a novel DV7S family member or is due to strain differences. The two DV104S1 rearrangements in F1D.73 are distinct as determined by differences in junctional diversity (data not shown). The in-frame TCR δ rearrangement in F1D.64 is presumed as the cell expresses a TCR δ chain.