Table 1.
Primary Activity and Frequency of GP33-specific CTL in Spleen after Immunization with H8-DC
| Single immunization | Repetitive immunization | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group | day 4 | day 8 | day 12 | day 16 | day 16 | |||||||
| 106 H8-DC | CTL activity | 35 ± 5 | 81 ± 7 | 25 ± 3 | 2 ± 1 | n.d. | ||||||
| CTLp frequency | 1/5,000 | 1/18,000 | 1/51,000 | 1/63,000 | n.d. | |||||||
| 105 H8-DC | CTL activity | n.d. | 74 ± 6 | n.d. | 2 ± 1 | 34 ± 5 | ||||||
| CTLp frequency | n.d. | 1/22,000 | n.d. | 1/73,000 | 1/83,000 | |||||||
| 104 H8-DC | CTL activity | 7 ± 2 | 59 ± 8 | 4 ± 1 | 1 ± 0 | n.d. | ||||||
| CTLp frequency | 1/15,000 | 1/45,000 | 1/60,000 | 1/119,000 | n.d. | |||||||
C57BL/6 mice were immunized intravenously at day 0 (single immunization) or at days 0 and 8 (repetitive immunization) with different numbers of H8-DC. At the indicated time point, effector spleen cells were prepared (three mice per group), and an overnight 51Cr-release assay was performed as detailed in Materials and Methods to determine DC-induced CTL activity and a limiting dilution assay was done to assess the CTLp frequency. CTL activity is shown as mean specific target cell lysis ± SD at an E/T ratio of 90:1. Data from one of three experiments are shown. n.d., Not done.