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. 1998 Oct 19;188(8):1445–1451. doi: 10.1084/jem.188.8.1445

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RT-PCR analysis of Ins-TRX transgene expression in various organs. RNA was extracted from the indicated organs of a transgenic mouse (line 90; Tg) and its TRX-negative littermate (non-Tg). 31 and 27 cycles of the PCR procedure were performed to detect the mRNA of human TRX (hTRX, top) and hprt (bottom), respectively. Samples were resolved by electrophoresis on 2% agarose gels and stained with an ethidium bromide solution.