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. 2000 May 1;191(9):1467–1476. doi: 10.1084/jem.191.9.1467

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Stimulation of H₂O₂ production by different doses of HP-NAP in human peripheral blood neutrophils (A) and monocytes (B). H₂O₂ was measured with the homovanillic acid method (reference 34). Very similar results were obtained by assaying the reduction of cytochrome c (not shown). Points are the average of five different experiments run in triplicate, and bars represent SD values. Symbols indicate: ▪, control; ♦, the data obtained with HP-NAP purified from H. pylori (average value of data obtained from two different preparations); all other points shown here and in the following pictures were obtained with highly purified recombinant HP-NAP expressed in B. subtilis: ▴, 0.6 μM HP-NAP; ▾, 1.2 μM HP-NAP; •, 3 μM HP-NAP; ○, 1 μM FMLP.