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. 2000 May 1;191(9):1555–1567. doi: 10.1084/jem.191.9.1555

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Homophilic adhesion of MCF-7 cells to E-cadherin-Fc mutants E31A, KEG30-32RDT, and E31D. Serial dilutations of wild-type and mutated E-cadherin and IgG1 were immobilized on plates coated with goat anti–human IgG antiserum. Homophilic adhesion assays with E-cadherin–expressing MCF-7 cells were performed in HBS with 1 mM CaCl₂, 50 mM glucose, and 0.1% BSA, as described in Materials and Methods. Results are shown as the mean percentage of MCF-7 cells bound ± 1 SD and are representative of at least two independent experiments in which each condition was performed in triplicate. (A) Homophilic adhesion to KEG30-32RDT (KEG). (B) Homophilic adhesion to E31A. (C) Homophilic adhesion to E31D.