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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Anal Biochem. 2007 Oct 1;373(1):121–128. doi: 10.1016/j.ab.2007.09.035

Figure 1.

Figure 1

[A] Schematic of the aptamer binding biodetection process. Briefly, either anti-protein antibody or biotinylated protein target was first immobilized onto magnetic beads. After incubation with the DNA aptamers, unbound aptamers were removed. The target-bound aptamer constructs were dissociated and the aptamers were analyzed in real time via either PCR or RCA. [B] Sequence of the 50nt ssDNA aptamer construct, comprised of a thrombin recognition domain, a poly-A linker spacer, and a short RCA primer.