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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Anal Biochem. 2007 Oct 1;373(1):121–128. doi: 10.1016/j.ab.2007.09.035

Figure 4.

Figure 4

Thrombin capture and real-time (rt) PCR detection. [A] Serial dilutions of thrombin were incubated with magnetic beads functionalized with an anti-thrombin antibody. Aptamers (2 nM) were subsequently added and detected by rt-PCR using a cycle threshold value of 300 RFU. [B] rt-PCR detection of thrombin in 10% bovine serum (v/v in binding buffer). Thrombin was diluted into 10% bovine serum, and incubated with Ab-functionalized beads. After briefly washing the beads, the aptamer (2 nM) was introduced and subsequently incubated for 1 hr. The beads were isolated and heat-dissociated aptamers were then detected by rt-PCR using a cycle threshold value of 300 RFU.